Rare sugars possess potential applications as low-calorie sweeteners. especially for anti-obesity and anti-diabetes. In this study. a fermentation biosystem based on the “DHAP-dependent aldolases strategy” was established for D-allulose and D-sorbose production from glycerol in endotoxin-free ClearColi BL21 (DE3). https://yeswellneses.shop/product-category/protein/